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What RNA element allows alphavirus RNA polymerase to generate negative strand?


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#1 Ahrenhase

Ahrenhase

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Posted 27 July 2015 - 06:07 AM

I'm trying to make a viral replicon particle and I'm not sure what elements I need to put at the 3' end so that the nsp1-nsp4 RNA-dependent RNA polymerase recognize the + strand in order to generate a - strand. 






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