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Chip Assay-Cell type?


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#1 Zohreh_fa

Zohreh_fa

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Posted Yesterday, 01:38 AM

Dear All,

 

I have a question regarding Chip - assay.

We want to use chip-assay to estimate target genes of a novel transcription factor which is assumed with a role in brain and nervous system.

 

My first question is, what kind of cell line I should start with.

Should I use a special neural cell line or any other kind of cell is proper for the work, for example lymphoblastoid cell lines?

 

Also, since this is the first time that I am going to do chip assay and there is no one in the lab with experience in the field,

I tried to find available kits for performing Chip in the lab.

For example, ChiP Kit (ab500) from abcam company or  Pierce Agarose ChIP Kit from Thermo Scientific.

Would you please kindly help me in this subject, if its better to use the commercially available kits or I should set it up in the lab manually?

 

Best Regards,

Zohreh

 




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