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SHSH5Y cells

MTT assay

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#1 Prake

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Posted 25 July 2015 - 01:53 PM

Hi Everyone,

 

I am using SHSY5Y cells to find out IC50 value of a metal using MTT, i suspect that presence of serum could interfere with metal uptake and decided to grow cells overnight in DMEM 10% FBS and then after oveernight growth i switched on to DMEM alone and incubated the cells at 37 C for one hour and treated @ various conc. Will manintaing cells in DMEM alone leads to poor uptake of metals or induces cells to undergo senescence or will it affect the outcome of the experiment.

Please advice,

Thanks-


Edited by Prake, 25 July 2015 - 01:54 PM.


#2 bob1

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Posted 26 July 2015 - 12:13 AM

It may well make them enter G0, but this should take some time.







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