Hi can someone please help me with a math problem?
I have a miniprep ( purified plasmid) concentration 225 ng/µl of a plasmid sized 2505 bp. I want to start a 10µl reaction that contains 0,001 µM DNA. Is my math correct:
so i found this :
DNA/RNA molarity Calculator:
M = w / MW
M: DNA molarity, in mol
w: DNA weight, in g
MW: DNA molecular weight, in g/mol
If only DNA length is given, the molecular weight is calculated as:
MW = DNA Length (bp) × DNA/RNA base weight
The approx. double strand DNA base weight is 650 Dalton, for single strand is 330, for RNA is 340 Dalton
therefore for 1 M reaction 2505*650=1628250 g/l??.
but I'm confused of how to continue my calculations?
Edited by bioduud, 17 July 2015 - 12:17 PM.