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How to prepare RNA sample to send for NGS?


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#1 Curtis

Curtis

    Metaller Scientist

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Excellent

Posted 14 July 2015 - 09:51 PM

In my previous lab we had a MySeq machine and after RNA extraction from virus we used RNAseq kits and loaded the sample into the machine. But in my current lab we don't have any machine and need to ship the samples overseas. I can add RNAse inhibitor and ship on dry ice, but I don't remember if we can add any inhibitor at all since NGS is sensitive to impurity. We don't want to send the virus itself, since it is an active biological sample. We have to extract RNA.




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