Hi I need help with preparing buffers. I have a pH range that i need to test my bacterium against. and for that i want to make buffers for example at each pH level.
The buffers i want to use are citric acid buffer for pH of 3.5, 4.5, 5.5, and 6.0.
MES buffer solution for making pH of 6.5
HEPES buffer solution for making pH of 7.0, 7.5, and 8.0 and to adjust pH i will be using HCl and NaOH
So for example to make citric acid buffer at pH of 3.5 (50mM) I need 0.7119% w/v citric acid and 0.3807% w/v sodium citrate how will i work this out in grams ?