Hello Everyone,
I new in this world of qRT-PCR and I need to quantify Influenza viral samples . Those are cell supernatants. For this as far as I could understood the method is the absolute quantification. I made a stand curve based on viral dilution then I ran the standard curve together with my samples. My question is how can I analyse these data? How to estimate the viral load?
I really appreciate any help.
THx
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Viral Quantification
Started by Richard.21, Jul 08 2015 08:31 AM
1 reply to this topic
#1
Posted 08 July 2015 - 08:31 AM
#2
Posted 08 July 2015 - 12:30 PM
Basically it comes down to you making a standard curve from the viral dilutions (perhaps Ct vs viral copy number in the dilution). Obtain the standard curve equation (it'll be something like x=a*y + , then use the Ct of your sample to estimate the number of viral copy number in that sample.