Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Viral Quantification


  • Please log in to reply
1 reply to this topic

#1 Richard.21

Richard.21

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 40 posts
0
Neutral

Posted 08 July 2015 - 08:31 AM

Hello Everyone, I new in this world of qRT-PCR and I need to quantify Influenza viral samples . Those are cell supernatants. For this as far as I could understood the method is the absolute quantification. I made a stand curve based on viral dilution then I ran the standard curve together with my samples. My question is how can I analyse these data? How to estimate the viral load? I really appreciate any help. THx

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,721 posts
567
Excellent

Posted 08 July 2015 - 12:30 PM

Basically it comes down to you making a standard curve from the viral dilutions (perhaps Ct vs viral copy number in the dilution). Obtain the standard curve equation (it'll be something like x=a*y + B), then use the Ct of your sample to estimate the number of viral copy number in that sample.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.