How do people establish isogenic clones from normal or immortalized human epithelial cells (non-transformed) when the cells are not clonogenic? I am referring to the ability of cells to form clones from single cells on plastic. I am not referring to cells being able to form colonies in soft agar which is a feature of transformed cells.
I want to introduce an inducible transgene into hTERT/p53 R175H immortalized cells then isolate isogenic clones but the cells will not grow as single cells. Will a richer media allow growth from single cells (ie add insulin or other growth factors)?Or do most people just work with polyclonal populations?