I want to extract DNA from alcohol preserved skin tissue from turtles.
1. Is there any treatment needed to the tissue to remove alcohol prior to extraction procedure?
2. I have tried Qiagen DNeasyBlood and tissue kit. I put the small tissue pieces in 180μL ATL lysis buffer with 20 μLProteinase k , 56°C overnight, even 48 hours but it does not lyse completely. How can I do complete lysis?
I am attaching my protocol. DNeasy-Blood--Tissue protocol.pdf 63.95KB 1743 downloads
Please someone please help me on this. This is my first post