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[qaiser15]

Hi !

 

I want to extract DNA from alcohol preserved skin tissue from turtles. 

 

1.     Is there any treatment needed to the tissue to remove alcohol prior to extraction  procedure?  

 

2.     I have tried Qiagen DNeasyBlood and tissue kit. I put the small tissue pieces in 180μL ATL lysis buffer with 20 μLProteinase k , 56°C                         overnight, even 48 hours but it  does not lyse completely. How can I do complete lysis?

         I am attaching my protocol.  DNeasy-Blood--Tissue protocol.pdf   63.95KB   1986 downloads

 

        Please  someone please help me on this. This is my first post

 

 

 

                                                                                                   With regards

                                                                                                     Qaiser