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Embryonic Tail Digestion Dilemma

Tail digestion Genotyping embryonic mouse tail tail digestion

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#1 mcoral346

mcoral346

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Posted 07 July 2015 - 12:58 PM

The tail of a developed mouse tail is very easy to digest overnight using this solution with added proteinase K. The tail is digested overnight at 56C and then the DNA is precipitated with a an approximate 1:1 ratio of Isopropyl.

 

 1x Tail Digestion Buffer

100mM Tris-HCL (pH 8.5)

5mM EDTA

0.2% SDS

200mM NaCl

 

However, 

I have been unable to find a good method for digesting embryonic mouse tails. After an overnight digestion the tail snip becomes a gelatinous goo and I cannot isolate the DNA from the goo efficiently when precipitating the DNA with Isopropyl.

Does anyone have any suggestions for a protocol I can try?







Also tagged with one or more of these keywords: Tail digestion, Genotyping, embryonic mouse tail, tail, digestion

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