The tail of a developed mouse tail is very easy to digest overnight using this solution with added proteinase K. The tail is digested overnight at 56C and then the DNA is precipitated with a an approximate 1:1 ratio of Isopropyl.
1x Tail Digestion Buffer
100mM Tris-HCL (pH 8.5)
I have been unable to find a good method for digesting embryonic mouse tails. After an overnight digestion the tail snip becomes a gelatinous goo and I cannot isolate the DNA from the goo efficiently when precipitating the DNA with Isopropyl.
Does anyone have any suggestions for a protocol I can try?