I´ve ordered SMC from human pulmonary artery. Even I´ve used the medium and all necessary supplements, ~98% of the cells didn´t attach to the flask bottom.
As the cells were super expensive I hoped for something like a miracle to happen ... so I waited 2 days until i found a few cells wich made it and attach.
Because I had nothing to lose I cultured them for more than a week and also tried to passage them which wordek fine...
Now I got some cell .... but I don´t think that they are SMC anymore. For me they look more than fibroblast.
In the end I also need fibroblast for my experiments... but are these (maybe) fibroblasts comparable with fibroblasts taken from pulmonary artery? Do they have same properties? Is this common that this happen ?
Are these fibroblasts?
Thank you in advance,