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# 1 in 2 dilution of a powder help please

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### #1 Gamewizard

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Posted 06 July 2015 - 07:40 AM

Hi ,  I basically need to make a 1 in 2 dilution of a solid material and i dont know how to do that.  The material is in powder form so i need to make it liquid so that i can use it for a microbiology test in the lab. So this is why i need to dilute it and i have decided to make a 1 in 2 dilution of it with sterile water. But I do not understand how much of the powder i will be putting in to how much water.

if i have 0.10 g  for example, how much would i need of this and how much of water to make a 1 in 2 dilution   ?

Help needed quick

### #2 mdfenko

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Posted 07 July 2015 - 03:33 AM

i think you want to solubilize to a final volume of 0.2ml.

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### #3 gvbdxz

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Posted 07 July 2015 - 04:07 AM

1 in 2 = 50% dilution which is usually 50g in 100 ml. So yeah as mdfenko said, 0.1 g in 0.2 ml

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### #4 pito

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Posted 07 July 2015 - 12:17 PM

A little bit of nitpicking here, but 0.1g to a total volume of 0.2ml, not "in" 0.2ml (it seems a bit like: adding 50ng in 0.2ml like you wrote it)

1 in 2 = 50% dilution which is usually 50g in 100 ml. So yeah as mdfenko said, 0.1 g in 0.2 ml

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### #5 gvbdxz

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Posted 08 July 2015 - 03:32 AM

A little bit of nitpicking here, but 0.1g to a total volume of 0.2ml, not "in" 0.2ml (it seems a bit like: adding 50ng in 0.2ml like you wrote it)

1 in 2 = 50% dilution which is usually 50g in 100 ml. So yeah as mdfenko said, 0.1 g in 0.2 ml

Yes you're right, thank you. 100 mg in water quantum satis 200 ul. Better?

Edited by gvbdxz, 08 July 2015 - 03:32 AM.

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### #6 Gamewizard

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Posted 08 July 2015 - 10:31 AM

Thank you everyone,  this makes sense now