Ok, so I was looking at buying a used desktop autoclave for about 1100 USD a couple of weeks back. It had one programable function, and with some help from helpful forumites here on BioForum - I was able to conclude that I should be able to use it for liquids.
however... my car just cost me that exact amount last week, so my means of investing in an autoclave just plummeted.
So - to my question: Just how important is it to have an autoclave if I want to work with bacterial transformation and DNA analysis? This is purely a hobby. Will the lack of an autoclave make it completely impossible for me to do any experiments? I've read through a few protocols, and every single one of them mentions at some point "..autoclave at XX degrees celcius for XX amount of time...".
Recently I found out I need to get a refrigerated micro centrifuge as well. I've discovered I should be able to modify the one I have though with a peltier based cooling system. But an autoclave... I dont see myselfing making a DIY version of that just yet.