taqman high CT's
Posted 06 August 2004 - 07:48 PM
The taqman I ran has very high CT's. Even RPL19 is very high. I checked the RNA concs again and everything seems fine. What else could be causing these high CT's? I am clueless. Thank you for any help
Posted 12 August 2004 - 03:40 AM
Posted 12 August 2004 - 04:14 AM
when our RT lost it's activity, i got high CTs, too. i found out that they correllated to not DNaseI digested DNA copys in the RNA prep. There always were 40-100 copys of DNA left in the sample, ever after digestion. They gave me positive signals in taqman assays at >38 cycles...
so maybe your sample doesn't hold rna or the RT reaction won't work, for whatever reasons...