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Bands are like a "w"


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#1 agiongo

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Posted 06 August 2004 - 12:28 PM

My box-PCR (bands range about 2000bp to 350bp) have shown bands like a "w". My agarose gel is 1,5% and I run it at 50mA and I use TBE.

#2 kant0008

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Posted 08 August 2004 - 05:09 PM

Hi,
normally strange band shape is to do with the gel, for example agarose not dissolving properly before you por so there are clumps, or setting slightly before you pour- again causing small clumps that would cause the sample to run unevenly. Try decreasing the agarose conc and make sure it all dissolves properly. It could theoretically be a problem with your power supply, but then you'd get a strange pattern all over your gel, not the same for all bands.




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