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Dilution and percentage problem


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3 replies to this topic

#1 Gamewizard

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Posted 23 June 2015 - 11:34 AM

Hi

 

so my question is basically on dilutions which I need to do for carrying out MIC

 

A 1 in 10 dilution converted to percentage is 0.01,   a 1 in 100 dilution is 0.001, however if I had a 1 in 10 dilution in my microtitre plate in well A1, in which I have added 1ml of bacterial diluent and 9ml of water, how much would I take from this column to the second column to make it 0.06% ?  and how much sterile water would I also add    

 

please help



#2 bob1

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Posted 24 June 2015 - 01:12 PM

Your maths is a bit out: 1:10 is 1/10 = 10% or 0.1 as an integer.



#3 Gamewizard

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Posted 25 June 2015 - 05:48 AM

Your maths is a bit out: 1:10 is 1/10 = 10% or 0.1 as an integer.

Sorry my bad. You are right. 0.01  is a 1 in 100 dilution not in 1 in 10 !   But do you know the answer to my question   >/ ?   

 

Or do you know how i can 0.06% concentration from 0.1% concentration. The final volume needs to be 1000 microliters of 0.06%      ?     :(   I need to know this !



#4 Phil Geis

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Posted 25 June 2015 - 06:32 AM

Concentration x volume = concentration x volume




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