I'm new to using Jurkat cells and wanted to ask whether transfection was necessary to study non-IL-2 characteristics of Jurkat cells. For example, can they express FOXP3? CTLA-4 or PD-1? or would I need to transfect these genes into the Jurkat cells prior to study?
Also, I've plated my cells out and was wondering if I need to continually split them or if I can just replace the media directly? I'm just worried since the cells are in suspension I'll aspirate the bulk of the cell when replacing the media.