I have got a question: At the moment I am trying to express a protein in Rosetta gami 2 (DE3) PLysS. After Induction with IPTG and purification with a Nickel column, I did a Bradford assay, which was positive, both in my wash- and elution-fractions. After that I did a SDS-PAGE with about 20 µg Protein per gel pocket, but found no protein on it after coomassie staining.
Does anyone have a good explanation for this?
Edited by VictoriaTrinkaus, 21 June 2015 - 09:46 AM.