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Detection of SMALL Peptide (5 amino acids in size)

Detectionprotein peptide short small

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#1 DoraTheExplorer

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Posted 21 June 2015 - 07:18 AM

Hello,

 

I'm still googling and searching through PubMed....

Does anyone know a method by which I can detect peptide fragment which are about 5 amino acids in length?

I guess Western Blot won't work for proteins that size?

 

Best regards and thanks in advance,

DoraTheExplorer



#2 mdfenko

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Posted 22 June 2015 - 05:31 AM

you can use tris-tricine sds-page (and immunoblot if you have an antibody against the peptide).

 

you can also separate the peptide and identify with hplc (especially if connected to a mass spectrometer).


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