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help with basic statistics to test reproducibility

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#1 avipasup



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Posted 18 June 2015 - 02:12 PM


I've been going really crazy :mad: with these statistical tests, been trying to understand this since two months with no luck.:confused:
What i have and what i want:
so, I have two independent experiments with 7 biological replicates each and 4 technical replicates for each of the biological replicates. Now i've been trying to get a suitable method to show that both the experiments have similar results and that the experiment is reproducible. 
What i did:
# Found the average of the four replicates
# Used shapiro wilks test to test the average from both the experiments for    normality.
#Used Welchs two sample t-test to test for statistical difference.
Did I do it right?? (btw Im a total noob at statistics :()
is there something else I can do here??
Thank you,

#2 DRT



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Posted 18 June 2015 - 02:56 PM

I think you have what’s called a “randomised complete block” with 7 treatments and 2 blocks.

A straight forward ANOVA will hopefully show no significant block effects.

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