Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How to make PCR analysis using the 22DDCT Method


  • Please log in to reply
1 reply to this topic

#1 Klaudek

Klaudek

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 17 June 2015 - 10:25 AM

Hey,

 

I am new in the PCR analysis word, I would like to do the data analysis using  22DDCT Method.

I have CT values and the analysis from the reference and control gene...and here I am lost.

How I should  procedure to  to start my data  analysis?

 

 



#2 Mad Researcher

Mad Researcher

    Newbie !!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 194 posts
12
Good

Posted 21 June 2015 - 02:21 AM

Hey,

 

I am new in the PCR analysis word, I would like to do the data analysis using  22DDCT Method.

I have CT values and the analysis from the reference and control gene...and here I am lost.

How I should  procedure to  to start my data  analysis?

 

 

 

This protocol will help you. It also gives a step by step example on how to calculate using the ddct method.

 

http://www.ncbi.nlm....pubmed/11846609


Edited by Mad Researcher, 21 June 2015 - 08:41 AM.

Cheers,

Mad Researcher




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.