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Can propidium iodide be used on WBC nuclei with cell membrane removed

propidium iodide wbc lysis

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#1 kimj

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Posted 16 June 2015 - 02:52 PM

I want to count WBC from whole blood. To remove the RBC I have to use a lysis buffer that removes the cell membrane, leaving just the nuclei intact. I would like to use propidium iodide to get a viability count on the nucleus. Will that work since PI permeates DNA instead of the cell membrane like trypan blue?

 



#2 Curtis

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Posted 16 June 2015 - 11:53 PM

PI-stained cells must be viewed immediately after staining. Otherwise everything turns orange. But RBC doesn't have nucleus. Or maybe I don't understand the question well.







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