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visualization of small peptide on tris-ricine sds page gel


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#1 marzieh

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Posted 16 June 2015 - 09:03 AM

hello

which material can be used to fix small peptides on  tris-ricine sds page gel before staining with coomassie blue?

(i know glutaraldehyde for this purpose. can you introduce me another material and the method to do fixation with that material please? )

thanks


Edited by marzieh, 16 June 2015 - 09:21 AM.


#2 mdfenko

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Posted 17 June 2015 - 04:11 AM

we usually just use the methanol/acetic acid that makes up the coomassie solution (fixation and staining in one step). we never seemed to have a problem with that method.

 

we often stained with silver and just followed our standard method.


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#3 marzieh

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Posted 22 June 2015 - 12:28 AM

using 18% resolving gel and then fixation of the peptide using formaldehyde before staining with coomassie blue, resulted in good resolution for detection of my about 5kDa peptide.






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