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Proper source of ampicillin for e.coli growth medium?


Best Answer bob1, 16 June 2015 - 12:11 AM

Yes it should be possible. You generally need the amp to be between 50 and 100 ug/ml of medium for selection. Note that you need to cool the medium to 55 (celsius) or less before adding the amp or you will inactivate it.

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#1 Dr. N00b

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Posted 15 June 2015 - 08:27 PM

Ok, so I want to modify some e.coli, and need an agar containing ampicillin in order to separate the wheat from the chaff. However, those pre-made kits from say, Bio-Rad - are incredibly expensive.

 

Would it be possible to make a regular growth medium from food grade agar and adding in ampicillin from another source, such as ampicillin capsules for fighting bacterial infections in pigeons?

 

Sincerely, N00b.


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#2 bob1

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Posted 16 June 2015 - 12:11 AM   Best Answer

Yes it should be possible. You generally need the amp to be between 50 and 100 ug/ml of medium for selection. Note that you need to cool the medium to 55 (celsius) or less before adding the amp or you will inactivate it.



#3 Dr. N00b

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Posted 16 June 2015 - 02:05 AM

Thanks Bob! Once again you come to my rescue. :)

 

I will try this out asap.

 

Sincerely, N00b.


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