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Livak method when there is no expression at all in the wild-type control


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#1 Shoshana

Shoshana

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Posted 15 June 2015 - 04:59 AM

What is the best way to show expression levels of a transgene that does not exist (and thus is not expressed at all) in the wild-type control?

 

With the Livak method, you are comparing expression in one set of samples to another set of samples to generate a fold change. But if the gene does not exist in the control, then it seems like fold change would be meaningless. So what's the best way to show that you have good expression of the transgene in your transformants?

 

Thanks!






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