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dna extraction


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4 replies to this topic

#1 vimesaba

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Posted 10 June 2015 - 11:00 PM

can anyone let me know about the volume of TE buffer which is needed to elute dna extracted from plasma by manual method?? will there be any difference in the quantity or quality of dna if TE buffer volume varies??



#2 Mad Researcher

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Posted 11 June 2015 - 05:37 AM

I use 50 uL of TE buffer for eluting my DNA.


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Mad Researcher

#3 vimesaba

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Posted 13 June 2015 - 02:28 AM

thank you.. but if i use 100 or 200 microlitre, will it have any impact?



#4 Mad Researcher

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Posted 13 June 2015 - 02:32 AM

I don't think so. I have read protocols where they use 200 ul of elution buffer for eluting the DNA. And if they need more they elute the DNA again with another 200 ul of elution buffer. So, in total they have 400 ul of DNA.


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#5 vimesaba

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Posted 13 June 2015 - 02:34 AM

fine... thank you.....






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