Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

PEG precipitation

  • Please log in to reply
No replies to this topic

#1 ixsix



  • Active Members
  • Pip
  • 24 posts

Posted 05 August 2004 - 07:29 PM

I combined cells and medium to purify aav with PEG 8000. In the final virus stork, lots of FCS was still detected by SDS. While the paper I looked for got quite pure virus(96%). AAV is resistant to chloroform.

Here is the flow chart. Does anyone have any opinion?

1 Homogenize cells by ultrasonic in start material(cells and medium combined)
2 10% chloroform and 1M nacl added
3 shaking 30 min
4 centrifuge and take the supernatant
5 add PEG 8000(10% w/v)
6 stand on the ice for an hour
7 centrifuge
8 collect the pellet and dissolve in pbs
9 add DNase I and RNase A for 30 min
10 add same volume of chloroform
11 centrifuge and take the supernatant

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.