I am not sure if i am doing it right.
I have 100 uM stock conc. of primers. I have made working solution of 10 uM conc. I further made working solution of 0.3 uM of my primers as i need to use 0.3 uM in my qPCR reaction.
I am taking 1 uL of the 0.3 uM primer but i guess i am taking way too much. How do i calculate the vol. of primer that i have to take for my final PCR reaction?
I appreciate your help in understanding this.