I am currently working on a dilution and need some help. Normally I use the C1V1=C2V2 equation when the diluent is DI water or something with no concentration.
However, I am working with trying to dilute down a sample with a concentration of say, 900ng/mL. The diluent I am using has a concentration of 20ng/mL. Is there an equation that I could use to do a 1:5 dilution of the sample using the diluent? I don't think I can use the C1V1=C2V2 because of the 20ng/mL diluent would throw off the end result and it wouldn't be a true 1:5 dilution.
Any help is appreciated.