Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Sec. structure in primers

Secondary structure Primers qPCR

  • Please log in to reply
1 reply to this topic

#1 Mad Researcher

Mad Researcher

    Newbie !!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 238 posts
13
Good

Posted 08 June 2015 - 09:52 AM

Hi,

 

I seem to have very weak to weak sec. structure in some of the primers i designed. Does/Will it affect my qPCR reaction in any way? Secondly, is there any way of getting rid of the sec. structures without re-designing the primers?

 

Thanks :)


Cheers,

Mad Researcher

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,478 posts
536
Excellent

Posted 08 June 2015 - 08:05 PM

Maybe is the best answer you will get with regard to secondary structure affecting your PCR.

 

You can use DMSO or betaine in conventional PCR to inhibit secondary structure fomation, but I don't know if this addition will affect qPCR.







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.