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Electrophoretic Mobility Band Shift Assay (EMSA)

EMSA Protein-DNA binding

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#1 Nurhikmah Mohd Sharif

Nurhikmah Mohd Sharif

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Posted 08 June 2015 - 12:55 AM

Hello everyone,

 

I want to ask some question regarding protein-DNA binding interaction assay. I am so new in this field. For my project, I have to do the EMSA. Can I run the EMSA without labelling the nucleic acid? And how do I want to perform the protein-DNA binding interaction without the labelled DNA? Anyone has the protocol of EMSA, could you please help me.

 

Thank you in advance.







Also tagged with one or more of these keywords: EMSA, Protein-DNA binding

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