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Electrophoretic Mobility Band Shift Assay (EMSA)

EMSA Protein-DNA binding

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#1 Nurhikmah Mohd Sharif

Nurhikmah Mohd Sharif

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Posted 08 June 2015 - 12:55 AM

Hello everyone,

 

I want to ask some question regarding protein-DNA binding interaction assay. I am so new in this field. For my project, I have to do the EMSA. Can I run the EMSA without labelling the nucleic acid? And how do I want to perform the protein-DNA binding interaction without the labelled DNA? Anyone has the protocol of EMSA, could you please help me.

 

Thank you in advance.







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