I need help about something really simple but it makes me wonder (for some reason
Ok, I am doing binding assay of protein and RNA, I use ATP in the mixture (25mM). Usually I use 2.5 ul of that ATP in mixture which is together with buffer total 40 ul (200ng/ul of RNA and protein each beginning concentration).
Now, my question is: if I use higher concentration of RNA and protein (for example total 10 ug) and total volume is 100 ul, how much of 25 mM ATP do I need in this mixture?
Thanks for the help!!
Edited by itmaybe, 08 June 2015 - 12:26 AM.