Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Proper agarose for bacteria colony?


  • Please log in to reply
4 replies to this topic

#1 Dr. N00b

Dr. N00b

    Professional Amateur

  • Awaiting Authorisation
  • PipPipPipPipPip
  • 44 posts
1
Neutral

Posted 07 June 2015 - 10:23 AM

Can I use any ordinary food grade agar for bacterial colonies like e.coli, or do I need some sort of special lab-grade agar?

 

Sincerely, N00b.


[url="http://www.drn00b.com"]Posted Image[/url]

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,706 posts
567
Excellent

Posted 07 June 2015 - 01:03 PM

Food grade should be fine.



#3 Dr. N00b

Dr. N00b

    Professional Amateur

  • Awaiting Authorisation
  • PipPipPipPipPip
  • 44 posts
1
Neutral

Posted 07 June 2015 - 03:26 PM

Food grade should be fine.

And this does not matter even if I plan on doing modifications to that very same e.coli, say making vectors?


[url="http://www.drn00b.com"]Posted Image[/url]

#4 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,706 posts
567
Excellent

Posted 07 June 2015 - 06:53 PM

No it doesn't matter, the agar is just a medium for the bacteria to grow on. The bacteria usually live in an evironment where there are a lot of contaminating DNA and other substances, but still grow happily. Most of the time DNA (which is the one you should be concerned about) is degraded by secreted DNases. To get DNA into E coli, you need to have certain chemical conditions and apply a shock (temperature or electrical) to the membrane that causes holes in it, so that the DNA can enter.

 

Check out how to prepare chemically competent cells (for heat shock) at http://openwetware.org/wiki/Protocols. I like the Top10 protocol, but the others work well too.



#5 Dr. N00b

Dr. N00b

    Professional Amateur

  • Awaiting Authorisation
  • PipPipPipPipPip
  • 44 posts
1
Neutral

Posted 08 June 2015 - 02:07 AM

No it doesn't matter, the agar is just a medium for the bacteria to grow on. The bacteria usually live in an evironment where there are a lot of contaminating DNA and other substances, but still grow happily. Most of the time DNA (which is the one you should be concerned about) is degraded by secreted DNases. To get DNA into E coli, you need to have certain chemical conditions and apply a shock (temperature or electrical) to the membrane that causes holes in it, so that the DNA can enter.

 

Check out how to prepare chemically competent cells (for heat shock) at http://openwetware.org/wiki/Protocols. I like the Top10 protocol, but the others work well too.

Thanks Bob! This is killer info. biggrin.png


[url="http://www.drn00b.com"]Posted Image[/url]




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.