I saw amplification in a negative control (not RT-, just not a sample where expression was expected) matches, or nearly matches, the melt curves for the expected product. But, there are very small differences in the shape/slope.
Negatives = two blue curves.
Negatives = two red curves with smallest peaks. Same primer pair as previous graph
Negatives = two blue curves with the highest peaks
[wishful thinking]: Would this be enough to rule them out as the product?
Thanks in advance for any info/advice...