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PCR efficiency

qPCR efficiency

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#1 Mad Researcher

Mad Researcher

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Posted 05 June 2015 - 06:39 AM



I got a PCR efficiency for all my targets and samples as 1. I did a quantitation analysis but it still calculated it as 1. I know that the R^2 value should be 0.9933 and slope should be -3.32 then by formula we get 1.


But as i didn't run a standard curve, how reliable is this efficiency result?


(lot of questions today).




Mad Researcher

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