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Melt curve analysis

Melt curve analysis qPCR

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#1 Mad Researcher

Mad Researcher

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Posted 05 June 2015 - 05:33 AM



I want to understand how do i analyze melt curve after my qPCR? What are the different curves?

I am just aware that melt curve analysis is done to check for secondary products or primer dimer formation.


I would appreciate your help in understanding the melt curve.




Mad Researcher

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