I re-precipitated my DNA today with Sodium Acetate. I followed a protocol that i found online and use 1/10th volume of 3M sodium acetate . Now, i saw another protocol where it says the final concentration of sodium acetate should be 0.3 M which i didn't. I got yield of DNA as negative values i.e -200.2 and -18.2 ng/ul.
My question is: should i have changed the concentration of sodium acetate to 0.3M and used. Why did i get negative values although weight can't be negative.
What protocol do you suggest?