Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

First ever project suggestions? - Starting genetic lab for hobby purposes.


  • Please log in to reply
40 replies to this topic

#31 Dr. N00b

Dr. N00b

    Professional Amateur

  • Awaiting Authorisation
  • PipPipPipPipPip
  • 44 posts
0
Neutral

Posted 29 June 2015 - 06:21 AM

Running it from inside a refrigerator is a no-go I think. Once I close the lid on that thing, it will be isolated from the rest of the environment in the refrigerator. Whatever heat is produced inside the chamber, the cold from the outside will not penetrate.

 

I just called up an electro engineer friend of mine and told him about my suggestion. 1. Drill a hole in each side of the centrifuge, 2. insert plastic tubing with the exact diameter of the hole, 3. attach a blowing fan to a peltier-cooled block, 4. attach thermostat inside chamber, and have it regulate the fan speed.

 

That way I can have the fan blow a steady stream of cold air through the chamber of the centrifuge, and the thermostat will lower or increase the fan speed depending on the chamber temperature.

 

However - how accurate must the temperature be? Exactly 4 C? or can it be like.. from +1 C to + 6 C?


[url="http://www.drn00b.com"]Posted Image[/url]

#32 pito

pito

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,464 posts
100
Excellent

Posted 29 June 2015 - 07:35 AM

well the key is to keep the cells cold because that way they are better in terms of competency and also because they are "weaker" so you need to keep them at 4°C in order not too damage them too much.


If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#33 Dr. N00b

Dr. N00b

    Professional Amateur

  • Awaiting Authorisation
  • PipPipPipPipPip
  • 44 posts
0
Neutral

Posted 01 July 2015 - 08:43 AM

Allright... so too low temperature= bad, too high temperature = also bad... :)

 

So, I've done some more thinking and this is what I am going to do:

 

Place small CPU fan inside lid of centrifuge, and one liquid CPU cooling system outside. Between these two I will sandwitch a heatsink, and a 400W peltier element.

A thermostat will regulate the speed of the liquid cooling system, which should allow me to control the temperature pretty accurately. :)

 

Would it be ok for me to go as low as say... 2 C, or as high as ... 5-6 C? Or is is 4 C exactly or I /fail?

 

the thermostat should be able to keep it pretty steady on 4 C though...

 

Going to order the parts off e-bay tonight. :) This will be an interesting project in may ways, both technically wth the equipment, as well as scientifically with the experiements... :D


[url="http://www.drn00b.com"]Posted Image[/url]

#34 pito

pito

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,464 posts
100
Excellent

Posted 01 July 2015 - 09:11 AM

Its fine.

It does not need to be exact 4°C , just keep it "cool", as you say: between 2 and 6°C.

As long as it does not freeze its ok (and keep it below 7°C or something like that).


If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#35 Phil Geis

Phil Geis

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 242 posts
19
Good

Posted 06 July 2015 - 02:27 AM

Reading these posts, I fear the hobby proceeds without a basic understanding of the elements that scientists tacitly and thoughtfully apply not only for the rigor and reliability of their wscience but more importantly here for the safety of the person doing the work (e.g. drilling holes in centrfuges).



#36 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,747 posts
304
Excellent

Posted 06 July 2015 - 06:14 AM

Styrofoam boxes, often used to ship dry ice containing samples, work very well. You can use two, a large and a small one, inside each other to improve the storage time.

These typically do not have to be vented. Find ones as thick as possible.



#37 pito

pito

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,464 posts
100
Excellent

Posted 06 July 2015 - 06:18 AM

I fear more for people doing stuff like this without posting what they are doing/want to do on a forum like this.

So far he has not done anything yet, he will be working with something very safe (lab E.coli strain and some plasmids).

 

The drilling of a hole, I do not see this as something very dangerous. People drill holes all the time, granted not in centrifuges, but it should not be a big issue.

 

 

Reading these posts, I fear the hobby proceeds without a basic understanding of the elements that scientists tacitly and thoughtfully apply not only for the rigor and reliability of their wscience but more importantly here for the safety of the person doing the work (e.g. drilling holes in centrfuges).

 


If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#38 Phil Geis

Phil Geis

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 242 posts
19
Good

Posted 06 July 2015 - 01:07 PM

Suggest we flatter ourselves if we presume we can establish safe and technically valid efforts via a fe discussions on a chat board.



#39 pito

pito

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,464 posts
100
Excellent

Posted 07 July 2015 - 05:01 AM

Suggest we flatter ourselves if we presume we can establish safe and technically valid efforts via a fe discussions on a chat board.

true,

 

but its not really rocket science he is doing...

 

Also: if you think like that, everyone at his/her home with a screwdriver, saw, drill or lets say an ikea packet should not start it because of safety issues...


If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#40 Ameya P

Ameya P

    Rervm Cognoscere Cavsas

  • Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 342 posts
35
Excellent

Posted 11 July 2015 - 04:36 AM

Suggest we flatter ourselves if we presume we can establish safe and technically valid efforts via a fe discussions on a chat board.

Dear Phil, 

 

I think the guy is doing something we ourselves have not done. We never tried to build a thermal cycler ourselves or even a electrophoresis box for that matter and that is simplest electronics in the lab. He must exercise caution when doing such things but is also not a 10 year old that needs parental supervision while doing all this. 

 

The joy is when you do everything yourself and we should encourage this habit, not only here but also in our respective labs. You could be the cautious one and point out all possible risks and that would also be great help.... but the IQ, OQ, PQ is too much over thinking....  let the boy have fun. 


NEW!!!! Would you donate your poop for a loved one? on CoffeeTableScience!!!! 

Image copyright: Adrian Koh SF.
Replication of this art is strictly prohibited without express permission of the artist


#41 Dr. N00b

Dr. N00b

    Professional Amateur

  • Awaiting Authorisation
  • PipPipPipPipPip
  • 44 posts
0
Neutral

Posted 25 July 2015 - 06:48 AM

I see there are some concerns concerning the microcentrifuge modification I am about to perform.

 

Completely understandable, however - unnecessary. smile.png A friend (electrical engineer) will assist me with the wiring, and I will be performing the rest myself. (I am told I can be quite handy... wink.png )

 

For those who are interested, I have attached a very quick illustration of what will be done in a PDF file:

Attached File  Centrifuge mod.pdf   272.08KB   168 downloads

 

Basically a low profile CPU fan will be sandwitched between the inner and out lid, sucking air through a hole in the middle, cooling it through a heat-sink, and circulating it back into the centrifuge chamber.

The heat-sink will be cooled by a 400W peltier element which in turn will be cooled by an exterior cooler. The peltier element will be controlled by a thermostat that continously monitors the chamber temperature.

 

I have yet to decide wether or not I want to use a liquid cooling system or an ordinary air-cooled one though...

An air-cooled one is less of a hassle to set up, but its big and clunky. A watercooled block is very low profile, but then again - it comes with a radiator with fan(s) on it... so.. yeah.. hehe wink.png

 

I've got all the parts now except for a power supply and the external cooler. I will be ordering these parts very soon.

 

Sincerely, N00b.

 

PS: when I start working on the centrifuge for "realz" as the gamers say - I will be posting allot more professionally looking walkthroughs on my quite recently developed www.DrN00b.com blog. (Big thanks to Ameya P for asking me to write about what I do. It was a good idea indeed. smile.png )


Edited by Dr. N00b, 25 July 2015 - 06:52 AM.

[url="http://www.drn00b.com"]Posted Image[/url]




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.