It might be very useful to spend some time in a working lab, learning the basic procedures in real life. Bench work doesn't always match the book and usually includes valuable information that no book has. Many research facilities have volunteer programs which could be a way for you to get started.
I understand that. However - I am not affiliated with any university or school, and I do not work in a profession that in any ways entails me rubbing shoulders with lab-people, if that is an acceptable term. I work in Aerospace with high precision tools. Far far away from any genetics lab.
I live in Norway - and in order to even set foot in a lab here you need to either be an enrolled student at an apropriate university, or a qualified lab-assistant or the likes, hired to actually work in a lab environment. If I were to e-mail a lab or two at a hospital or university or what not - I'd most likely be met with silence, or a polite reply that "sorry, but we are unable to offer you any such assistance".
The other day I made a few calls to the local hospital(s) that had suitable labs. I asked about lab strain e.coli and whether or not they had any. I was put through from phone to phone until I finally met someone who could not help me. They had lab strain e.coli on ice though.. They just couldnt give or sell any to me.
So... to the point: I am completely on my own here, and I will continue to be on my own.
I do however - thanks to a very friendly and helpful customer relations worker at Bio-Rad Life Systems - have a customer account with them.
Which brings me to the reason why I looked through my posts and started writing here.
If I am going to get any e.coli, I need to buy it from an actual provider. Bio-Rad is such a provider.
Now, this stuff is expensive... And I am thinking, which e.coli could I buy from them and "keep alive" in a large, or several colonies by myself to have a relatively endless supply of them?
So far I have been recommended these two products:
Subcloning Efficiency™ DH5α™ Competent Cells
One Shot® TOP10 Chemically Competent E. coli
the first one is obviously the most affordable to me, but at my current level I am unable to distinguish the different versions from eachother to find the most suitable allrounder.
What I want to do is created plasmid vectors, insert separated gene into vector, infuse e.coli with this vector and have them express - say a green fluoresence protein. Which product do I go for?
I know I can buy ready-to-go plasmid vectors, but I want to learn how to do it myself... so..
Oh, and welcome to the world's smallest lab. (see attachments).
It is starting to look like something. I still need to get an autoclave amongst other things. I will continue to build on this during the next few months. I do not own a house, so this is all the space I've got to work with. I got inspired by the bio-hacking community and went all out.
Sincerely, Dr. N00b.