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Is cultured cell reuse possible?


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#1 wswr

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Posted 03 June 2015 - 04:24 PM

I know this is not a deep question, but I would like the following to be clarified. I have selected a colony of BL21(DE3) picked up from LB-agar plate to inoculate a liquid medium an hour ago. But I realized I screwed up and would like to do it. The LB-agar plate has been left in room temperature for an hour or so. Can I select another colony form that plate again? Could I put the LB-agar plate back into the incubator before inoculating again? Thank you in advance.



#2 bob1

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Posted 03 June 2015 - 06:04 PM

It should be fine, E. coli's growth at room temperature is only slowed, not completely inhibited.






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