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Count cell bactria using haemacytometer

microbiology bacteria

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#1 PuspasriNT

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Posted 02 June 2015 - 08:17 AM

i need your help so bad
i get confused with my task
if i want to count cell of bacteria using haemocytometer, should i count the square in the middle too?
and when i count total cell should i use formula :

count total cell x dilution factor/ total of squre x 10.000 cell/ml
or

(0,004mm = total volume each square)

if i use 5 square >> 5 x 0,004 mm = 0,02 mm (total volume)

so >> total cell / 0,02 mm x 1000 m3(cubic)

???

please help me


Edited by PuspasriNT, 02 June 2015 - 08:19 AM.


#2 pito

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Posted 02 June 2015 - 08:54 AM

It is pretty well explained here: http://en.wikipedia....i/Hemocytometer

Did you check it?

Keep in mind: not all counting chambers use the same measurements for the squares! So you do need to keep this in mind.

 

also check here: http://www.hemocytom...er-square-size/

and here: http://celeromics.co...uer-chamber.pdf

 

If this does not help, let us know, but I think with this information you can figure it out yourself.

 

 

About the 10.000cells/ml, not sure where you get this number?

 

 

i need your help so bad
i get confused with my task
if i want to count cell of bacteria using haemocytometer, should i count the square in the middle too?
and when i count total cell should i use formula :

count total cell x dilution factor/ total of squre x 10.000 cell/ml
or

(0,004mm = total volume each square)

if i use 5 square >> 5 x 0,004 mm = 0,02 mm (total volume)

so >> total cell / 0,02 mm x 1000 m3(cubic)

???

please help me

 


If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.






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