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Comparing DNA fragments using restriction map


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#1 starsun24

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Posted 01 June 2015 - 09:06 AM

I performed a restriction digest using Hind III to cut plasmid pUC19 and lambda DNA. The results were analyzed using gel electrophoresis.I created a standard curve and obtained the sizes of the pUC19 and lambda fragments and I'm supposed to compare that to the restriction map. Im completely confused as to how to do this confusion. Please help.

 I've attached a picture of the restriction maps of pUC19 and lambda DNA fragments to the post. How do I use this to compare it to my own sizes? For example, my pUC19 fragment traveled 30mm and is 3.1 kb, but how do i compare that to the published size to see if its right?? 

 

dcsdc.jpg


Edited by starsun24, 01 June 2015 - 09:53 AM.


#2 bob1

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Posted 01 June 2015 - 02:58 PM

You don't need to compare migration distances, only pattern...How many fragments would HindIII produce if it cut pUC19 (or lambda), how big would each fragment be?






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