I performed a restriction digest using Hind III to cut plasmid pUC19 and lambda DNA. The results were analyzed using gel electrophoresis.I created a standard curve and obtained the sizes of the pUC19 and lambda fragments and I'm supposed to compare that to the restriction map. Im completely confused as to how to do this confusion. Please help.
I've attached a picture of the restriction maps of pUC19 and lambda DNA fragments to the post. How do I use this to compare it to my own sizes? For example, my pUC19 fragment traveled 30mm and is 3.1 kb, but how do i compare that to the published size to see if its right??
Edited by starsun24, 01 June 2015 - 09:53 AM.