I asked something similar in molecular but I think I should ask it here. I had 150ul of PCR reaction which I used QIAquick-pcr-purification kit on. I did a 1% agarose gel to check to make sure I had right band before I did the purification. Now I have 50ul and my next step is the restriction digest with NEB Agel HF and Hindlll HF. My questions are how of the 50ul should I use and can use both enzymes at the same time?