I am using Zymogen RNA extraction kit for extracting RNA from brain samples. I have the following questions regarding RNA extraction:
1) I am using a pestle to crush the tissue and it is recommended by the manufacturer to use the Lysis buffer while i crush the tissue. But while using the lysis buffer for crushing the tissue there is foam generation and its difficult to transfer the mixture to a new tube. Any suggestion what can be used alternatively instead of the lysis buffer while crushing the tissue and to avoid foaming?
2) There is a DNAse treatment included in the protocol which i did and i again did another DNAse treatment. Will that harm my sample in any ways?