this is the first time i've done PCR and after pcr i ran the DNA on 0.8% agarose without EtBr but i did not get any bands. the concentrations i got were 38.6 and 19.2 for my samples and the nanodrop 260/280 ratios were 2.32 and 1.96 respectively. the concentrations before doing PCR and pcr product purification with sigma-aldrich PCR clean-up kit were 284.7 [260/280= 1.40] and 227.7 [260/280= 1.44] following are the pcr mix concentrations (50uL) buffer; 8.44, MgCl2; 8.44, dNTPs; 6.75, forward and reverse primers; 1uL each, template; 1uL, Taq polymerase; 0.67 uL[all values in uL] and temperature settings: initial; 94
ͦ c, 94*c, annealing; 55*c, extention: 72*c, final extention: 72*c, hold at 4*c.
kindly help me out with this. what might be the reason that i m not getting bands after pcr and purification??