I am seeking some advice on performing an antibacterial assay. What I plan to do as a preliminary experiment is to culture some E.coli to mid-exponential phase. Incubate it with my chosen antibacterial peptide for a period of time and plate them out to determine the CFU counts. My question is:
- What strain of E. coli is normally used for antibacterial assays? ( We only have DH5alpha available, not sure if that is suitable)
- How do I go about determining the growth phase of E.coli. ( I have read that exponential growing bacteria have an absorbance of 0.5 at OD 600).
Any comments will be much appreciated