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Antibacterial Assay


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#1 tofuj

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Posted 25 May 2015 - 03:19 AM

Hi all! 

 

I am seeking some advice on performing an antibacterial assay. What I plan to do as a preliminary experiment is to culture some E.coli to mid-exponential phase. Incubate it with my chosen antibacterial peptide for a period of time and plate them out to determine the CFU counts. My question is: 

 

- What strain of E. coli is normally used for antibacterial assays? ( We only have DH5alpha available, not sure if that is suitable)

- How do I go about determining the growth phase of E.coli. ( I have read that exponential growing bacteria have an absorbance of 0.5 at OD 600). 

 

Any comments will be much appreciated smile.png



#2 bob1

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Posted 25 May 2015 - 12:45 PM

Exponential growth can be determined by plotting the OD600 over time, you'll soon see when the exponential growth happens.

 

No idea if DH5a is suitable for your assay though.






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