I received my primers today (in a plate) and the datasheet specified to add 100 ul of water to get a 100 micromolar solution.
Now, i want to use a 0.3 micromolar solution in my qPCR so how do i dilute it further. I want a final volume of 25 ul.
I could dilute my 100 micromolar solution to 10 or 5 micromolar but then again whenever i have to run a qPCR i have to dilute it again. Any solution how to dilute to get a final conc. of 0.3 or 0.4 micromolar solution to be used with qPCR?
Edited by Mad researcher, 20 May 2015 - 11:10 AM.