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Plasma cortisol/corticosterone level analysis using qPCR

plasma corticosterone cortisol qPCR

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#1 Mad Researcher

Mad Researcher

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Posted 15 May 2015 - 01:30 AM



This question may sound really stupid but i wanted to know if we can analyze plasma cortisol/corticosterone levels from Plasma samples using qPCR? I am aware that we can analyze for microRNAs using qPCR but i want to measure the changes in cortisol/corticosterone levels to determine the stress level of the animal using qPCR.


Is this possible? If yes, could you please direct me to some papers/articles/methods for the same.


Cheers !!


Mad Researcher

#2 bob1


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Posted 15 May 2015 - 06:54 PM

You certainly won't get a direct read-out as qPCR only works for things that can be turned into DNA...

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